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MSCV-PIG-N3FLAG-TAF12
(Plasmid #105593)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 105593 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pMSCV-PGK-puro-IRES-GFP
  • Vector type
    Mammalian Expression, Retroviral
  • Selectable markers
    Puromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    full length TAF12 with silent mutations, making it resistant to TAF12 shRNA#364
  • Species
    M. musculus (mouse)
  • Mutation
    silent mutations to make it resistant to the targeting of TAF12 shRNA#364
  • Entrez Gene
    Taf12 (a.k.a. 20kDa, 2810422D08Rik, Taf2J)
  • Promoter MSCV-LTR
  • Tag / Fusion Protein
    • 3*FLAG (N terminal on insert)

Cloning Information

  • Cloning method Ligation Independent Cloning
  • 5′ sequencing primer CCC TTG AAC CTC CTC GTT CGA CC
  • 3′ sequencing primer GAG ACG TGC TAC TTC CAT TTG TC
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    MSCV-PIG-N3FLAG-TAF12 was a gift from Christopher Vakoc (Addgene plasmid # 105593 ; http://n2t.net/addgene:105593 ; RRID:Addgene_105593)

  • For your References section:

    A TFIID-SAGA Perturbation that Targets MYB and Suppresses Acute Myeloid Leukemia. Xu Y, Milazzo JP, Somerville TDD, Tarumoto Y, Huang YH, Ostrander EL, Wilkinson JE, Challen GA, Vakoc CR. Cancer Cell. 2018 Jan 8;33(1):13-28.e8. doi: 10.1016/j.ccell.2017.12.002. 10.1016/j.ccell.2017.12.002 PubMed 29316427
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