pGEMHE-mCherry-mTrim21ΔRING-Box
(Plasmid #105523)

• Purpose: T7 promotor drives in vitro transcription of mCherry-tagged mouse TRIM21 with deleted RING-box mRNA
• Depositing Lab: Melina Schuh
• Publication: Clift et al Cell. 2017 Nov 13. pii: S0092-8674(17)31255-2. doi: 10.1016/j.cell.2017.10.033.

Backbone

• Vector backbone: pGEMHE
• Backbone size w/o insert (bp): 3807
• Total vector size (bp): 4827
• Vector type: in vitro Transcription

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: TRIM21
• Alt name: Ro52
• Alt name: Ssa1
• Species: M. musculus (mouse)
• Insert Size (bp): 1020
• Mutation: deletion of RING domain (aa124-462)
• GenBank ID: NM_001082552.2
• Entrez Gene: Trim21 (a.k.a. Ro52, Ssa1)
• Promoter: T7
• Tag / Fusion Protein:
  • mCherry (N terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: EcoRI (not destroyed)
• 3′ cloning site: NotI (not destroyed)
• 5′ sequencing primer: CCCCGTAATGCAGAAGAAGA
• 3′ sequencing primer: TCGGGTGTTCTTGAGGCTGG

Resource Information

• Supplemental Documents:
  • pGEMHE-mCherry-mTrim21deltaRING-Box.gb
• A portion of this plasmid was derived from a plasmid made by: Trim sequence was originally cloned by Leo James/William McEwan also MRC Laboratory of Molecular Biology
• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Takara Bio Limited Use Label License (formerly Clontech)
• Industry Terms:
  • Not Available to Industry

Depositor Comments

The sequence contains an E318K in mTrim21. This mutation does not affect plasmid function as described in the associated publication.