pET-NDH6
(Plasmid
#105503)
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PurposeExpression of an N-terminally truncated form of Drosophila topoisomerase I with a C-terminal 6His tag
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 105503 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepET
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameNDH6
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Tag
/ Fusion Protein
- 6xHis (C terminal on insert)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pET-NDH6 was a gift from James Kadonaga (Addgene plasmid # 105503 ; http://n2t.net/addgene:105503 ; RRID:Addgene_105503) -
For your References section:
A simple and versatile system for the ATP-dependent assembly of chromatin. Khuong MT, Fei J, Cruz-Becerra G, Kadonaga JT. J Biol Chem. 2017 Nov 24;292(47):19478-19490. doi: 10.1074/jbc.M117.815365. Epub 2017 Oct 5. 10.1074/jbc.M117.815365 PubMed 28982979
