pISH-Olfr16
(Plasmid
#105486)
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Purposeto generate a riboprobe for in situ hybridazation
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 105486 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepGEM-T
- Backbone size w/o insert (bp) 3000
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Vector typeto ganerate riboprobe
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
Cloning Information
- Cloning method Unknown
- 5′ sequencing primer M13-for
- 3′ sequencing primer M13-Reverse (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Linearize with NcoI, use SP6 RNA polymerase for IVT.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pISH-Olfr16 was a gift from Peter Mombaerts (Addgene plasmid # 105486 ; http://n2t.net/addgene:105486 ; RRID:Addgene_105486) -
For your References section:
The beta2-adrenergic receptor as a surrogate odorant receptor in mouse olfactory sensory neurons. Omura M, Grosmaitre X, Ma M, Mombaerts P. Mol Cell Neurosci. 2014 Jan;58:1-10. doi: 10.1016/j.mcn.2013.10.010. Epub 2013 Nov 6. 10.1016/j.mcn.2013.10.010 PubMed 24211702