pISH-Gucy1b2-probe1
(Plasmid
#105454)
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Purposeto generate a riboprobe for in situ hybridazation
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 105454 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepGEM-Teasy
- Backbone size w/o insert (bp) 3015
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Vector typeto ganerate riboprobe
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameGucy1b2
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SpeciesM. musculus (mouse)
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Insert Size (bp)1090
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Entrez GeneGucy1b2 (a.k.a. B930025B03, GC-SB2)
Cloning Information
- Cloning method Unknown
- 5′ sequencing primer M13-for
- 3′ sequencing primer M13-Reverse (Common Sequencing Primers)
Resource Information
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Article Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Linearize with NcoI, use SP6 RNA polymerase for IVT.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pISH-Gucy1b2-probe1 was a gift from Peter Mombaerts (Addgene plasmid # 105454 ; http://n2t.net/addgene:105454 ; RRID:Addgene_105454) -
For your References section:
Trpc2-expressing sensory neurons in the mouse main olfactory epithelium of type B express the soluble guanylate cyclase Gucy1b2. Omura M, Mombaerts P. Mol Cell Neurosci. 2015 Mar;65:114-24. doi: 10.1016/j.mcn.2015.02.012. Epub 2015 Feb 19. 10.1016/j.mcn.2015.02.012 PubMed 25701815
