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PurposeCation channelrhodopsin ChrimsonR fused to mRuby2 fluorophore and targeted to the neuronal soma and proximal dendrites
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 105447 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepCAGGS
- Backbone size w/o insert (bp) 4813
- Total vector size (bp) 6793
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameChrimsonR-mRuby2-ST
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Insert Size (bp)1980
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MutationK176R in Chrimson
- Promoter pCAGGS
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Tags
/ Fusion Proteins
- mRuby2
- soma targeting motif
Cloning Information
- Cloning method Ligation Independent Cloning
- 5′ sequencing primer Custom
- 3′ sequencing primer Custom (Common Sequencing Primers)
Resource Information
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Article Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pCAGGS-ChrimsonR-mRuby2-ST was a gift from Hillel Adesnik (Addgene plasmid # 105447 ; http://n2t.net/addgene:105447 ; RRID:Addgene_105447) -
For your References section:
Three-dimensional scanless holographic optogenetics with temporal focusing (3D-SHOT). Pegard NC, Mardinly AR, Oldenburg IA, Sridharan S, Waller L, Adesnik H. Nat Commun. 2017 Oct 31;8(1):1228. doi: 10.1038/s41467-017-01031-3. 10.1038/s41467-017-01031-3 PubMed 29089483
Map uploaded by the depositor.
