pCAGGS-ChrimsonR-mRuby2-ST
(Plasmid #105447)

• Purpose: Cation channelrhodopsin ChrimsonR fused to mRuby2 fluorophore and targeted to the neuronal soma and proximal dendrites
• Depositing Lab: Hillel Adesnik
• Publication: Pegard et al Nat Commun. 2017 Oct 31;8(1):1228. doi: 10.1038/s41467-017-01031-3.

Backbone

• Vector backbone: pCAGGS
• Backbone size w/o insert (bp): 4813
• Total vector size (bp): 6793
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: ChrimsonR-mRuby2-ST
• Insert Size (bp): 1980
• Mutation: K176R in Chrimson
• Promoter: pCAGGS
• Tags / Fusion Proteins:
  • mRuby2
  • soma targeting motif

Cloning Information

• Cloning method: Ligation Independent Cloning
• 5′ sequencing primer: Custom
• 3′ sequencing primer: Custom

Resource Information

• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry