tol2-lyzC-miR-100-1-dendra2
(Plasmid
#105438)
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Purposeoverexpression of microRNA and Dendra2 in Zebrafish
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 105438 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backboneTol2
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Vector typeZebrafish
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)NEB Stable
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namemiR-100-1 Dendra2
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SpeciesD. rerio (zebrafish)
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Entrez Genemir100-1 (a.k.a. mirn100-1)
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Tag
/ Fusion Protein
- N/A
Cloning Information
- Cloning method Ligation Independent Cloning
- 5′ sequencing primer Unknown (Common Sequencing Primers)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
tol2-lyzC-miR-100-1-dendra2 was a gift from Qing Deng (Addgene plasmid # 105438 ; http://n2t.net/addgene:105438 ; RRID:Addgene_105438) -
For your References section:
Phenotypical microRNA screen reveals a noncanonical role of CDK2 in regulating neutrophil migration. Hsu AY, Wang D, Liu S, Lu J, Syahirah R, Bennin DA, Huttenlocher A, Umulis DM, Wan J, Deng Q. Proc Natl Acad Sci U S A. 2019 Aug 26. pii: 1905221116. doi: 10.1073/pnas.1905221116. 10.1073/pnas.1905221116 PubMed 31451657