VAChT-SNAP
(Plasmid
#105318)
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PurposeVAChT tagged with SNAP
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 105318 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepcDNA3.1
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Vector typeMammalian Expression
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Selectable markersNeomycin (select with G418)
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameVAChT
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Alt nameVesicular acetylcholine transporter
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SpeciesR. norvegicus (rat)
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Entrez GeneSlc18a3 (a.k.a. VACht, rVAT)
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Tag
/ Fusion Protein
- SNAP tag
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
VAChT-SNAP was a gift from Justin Taraska (Addgene plasmid # 105318 ; http://n2t.net/addgene:105318 ; RRID:Addgene_105318) -
For your References section:
Semisynthetic fluorescent pH sensors for imaging exocytosis and endocytosis. Martineau M, Somasundaram A, Grimm JB, Gruber TD, Choquet D, Taraska JW, Lavis LD, Perrais D. Nat Commun. 2017 Nov 10;8(1):1412. doi: 10.1038/s41467-017-01752-5. 10.1038/s41467-017-01752-5 [pii] PubMed 29123102