pmCherry Tensin3
(Plasmid #105301)

• Purpose: To study localization and function of Tensin 3 with EGFP Tensin1
• Depositing Lab: Kenneth Yamada
• Publication: Clark et al J Cell Biochem. 2010 Mar 1;109(4):808-17. doi: 10.1002/jcb.22460.

Backbone

• Vector backbone: pmCherry NBC1
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Tensin3
• Alt name: TNS3
• Alt name: NM_022748
• Species: H. sapiens (human)
• Insert Size (bp): 4300
• Entrez Gene: TNS3 (a.k.a. TEM6, TENS1)
• Tag / Fusion Protein:
  • mCherry (N terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: EcoR1 (unknown if destroyed)
• 3′ cloning site: Sal1 (unknown if destroyed)

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Takara Bio Limited Use Label License (formerly Clontech)
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Human tensin 1 was from Dr. Katherine Clark of University of Leicester, UK

Note: The TNS3 insert contains a C1276Y mutation compared to the listed reference sequence. The depositor has confirmed that this does not affect plasmid function.