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Addgene

pEGFP Tensin2
(Plasmid #105298)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 105298 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pEGFP-C1
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Tensin2
  • Alt name
    TNS2
  • Alt name
    NM_198316
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    3900
  • Mutation
    lacks N-terminal C1 domain
  • Entrez Gene
    TNS2 (a.k.a. C1-TEN, C1TEN, TENC1)
  • Tag / Fusion Protein
    • EGFP (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoR1 (unknown if destroyed)
  • 3′ cloning site Xba1 (unknown if destroyed)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pEGFP Tensin2 was a gift from David Critchley & Kenneth Yamada (Addgene plasmid # 105298 ; http://n2t.net/addgene:105298 ; RRID:Addgene_105298)

  • For your References section:

    Tensin 2 modulates cell contractility in 3D collagen gels through the RhoGAP DLC1. Clark K, Howe JD, Pullar CE, Green JA, Artym VV, Yamada KM, Critchley DR. J Cell Biochem. 2010 Mar 1;109(4):808-17. doi: 10.1002/jcb.22460. 10.1002/jcb.22460 PubMed 20069572
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