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Addgene

HR_G3BP1-V5-APEX2-GFP
(Plasmid #105284)

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 105284 Standard format: Plasmid sent in bacteria as agar stab 1 $85

Backbone

  • Vector backbone
    HR120PA-1
  • Backbone manufacturer
    Systems Biosciences
  • Backbone size w/o insert (bp) 2668
  • Total vector size (bp) 12248
  • Modifications to backbone
    inserted PGK-DTA-pA negative selection cassette downstream of 3' arm of homology.
  • Vector type
    human homologous recombination donor
  • Selectable markers
    Puromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    G3BP1 homology arms flanking V5-APEX2-GFP and EF1a-puro selection cassette
  • Species
    H. sapiens (human); soybean
  • Insert Size (bp)
    8004
  • Entrez Gene
    G3BP1 (a.k.a. G3BP, HDH-VIII)

Cloning Information

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    HR_G3BP1-V5-APEX2-GFP was a gift from Eugene Yeo (Addgene plasmid # 105284 ; http://n2t.net/addgene:105284 ; RRID:Addgene_105284)
  • For your References section:

    Context-Dependent and Disease-Specific Diversity in Protein Interactions within Stress Granules. Markmiller S, Soltanieh S, Server KL, Mak R, Jin W, Fang MY, Luo EC, Krach F, Yang D, Sen A, Fulzele A, Wozniak JM, Gonzalez DJ, Kankel MW, Gao FB, Bennett EJ, Lecuyer E, Yeo GW. Cell. 2018 Jan 25;172(3):590-604.e13. doi: 10.1016/j.cell.2017.12.032. 10.1016/j.cell.2017.12.032 PubMed 29373831