HR_G3BP1-V5-APEX2-GFP
(Plasmid #105284)

• Purpose: homologous recombination donor top insert V5-APEX2-GFP into the C-terminus of the human G3BP1 locus
• Depositing Lab: Eugene Yeo
• Publication: Markmiller et al Cell. 2018 Jan 25;172(3):590-604.e13. doi: 10.1016/j.cell.2017.12.032.

Backbone

• Vector backbone: HR120PA-1
• Backbone manufacturer: Systems Biosciences
• Backbone size w/o insert (bp): 2668
• Total vector size (bp): 12248
• Modifications to backbone: inserted PGK-DTA-pA negative selection cassette downstream of 3' arm of homology.
• Vector type: human homologous recombination donor
• Selectable markers: Puromycin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: G3BP1 homology arms flanking V5-APEX2-GFP and EF1a-puro selection cassette
• Species: H. sapiens (human); soybean
• Insert Size (bp): 8004
• Entrez Gene: G3BP1 (a.k.a. G3BP, HDH-VIII)

Cloning Information

• Cloning method: Gibson Cloning
• 5′ sequencing primer: M13 (-40) Forward
• 3′ sequencing primer: M13R

Resource Information

• Articles Citing this Plasmid:
  • 2 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry