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Purposehomologous recombination donor top insert V5-APEX2-GFP into the C-terminus of the human G3BP1 locus
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 105284 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backboneHR120PA-1
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Backbone manufacturerSystems Biosciences
- Backbone size w/o insert (bp) 2668
- Total vector size (bp) 12248
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Modifications to backboneinserted PGK-DTA-pA negative selection cassette downstream of 3' arm of homology.
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Vector typehuman homologous recombination donor
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Selectable markersPuromycin
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameG3BP1 homology arms flanking V5-APEX2-GFP and EF1a-puro selection cassette
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SpeciesH. sapiens (human); soybean
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Insert Size (bp)8004
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Entrez GeneG3BP1 (a.k.a. G3BP, HDH-VIII)
Cloning Information
- Cloning method Gibson Cloning
- 5′ sequencing primer M13 (-40) Forward
- 3′ sequencing primer M13R (Common Sequencing Primers)
Resource Information
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Articles Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
HR_G3BP1-V5-APEX2-GFP was a gift from Eugene Yeo (Addgene plasmid # 105284 ; http://n2t.net/addgene:105284 ; RRID:Addgene_105284) -
For your References section:
Context-Dependent and Disease-Specific Diversity in Protein Interactions within Stress Granules. Markmiller S, Soltanieh S, Server KL, Mak R, Jin W, Fang MY, Luo EC, Krach F, Yang D, Sen A, Fulzele A, Wozniak JM, Gonzalez DJ, Kankel MW, Gao FB, Bennett EJ, Lecuyer E, Yeo GW. Cell. 2018 Jan 25;172(3):590-604.e13. doi: 10.1016/j.cell.2017.12.032. 10.1016/j.cell.2017.12.032 PubMed 29373831