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pRS406-GFP-SNC1(L96V)
(Plasmid #105278)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 105278 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pRS406
  • Backbone manufacturer
    Stratagene
  • Backbone size w/o insert (bp) 4384
  • Total vector size (bp) 5965
  • Vector type
    Yeast Expression
  • Selectable markers
    URA3

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    Snc1
  • Species
    S. cerevisiae (budding yeast)
  • Mutation
    Leucine 96 changed to Valine, intron removed
  • Entrez Gene
    SNC1 (a.k.a. YAL030W)
  • Promoter TPI1
  • Tag / Fusion Protein
    • GFP (N terminal on insert)

Cloning Information

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    Original pRS406-GFP-SNC1 plasmid from Nava Segev lab

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pRS406-GFP-SNC1(L96V) was a gift from Benjamin Glick (Addgene plasmid # 105278 ; http://n2t.net/addgene:105278 ; RRID:Addgene_105278)

  • For your References section:

    Budding Yeast Has a Minimal Endomembrane System. Day KJ, Casler JC, Glick BS. Dev Cell. 2018 Jan 8;44(1):56-72.e4. doi: 10.1016/j.devcel.2017.12.014. Epub 2018 Jan 8. 10.1016/j.devcel.2017.12.014 PubMed 29316441
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