YIplac211-VPS8-mCherry2Bx6
(Plasmid #105273)

• Purpose: Plasmid for pop-in/pop-out replacement of Vps8 with Vps8-mCherry2Bx6.
• Depositing Lab: Benjamin Glick
• Publication: Day et al Dev Cell. 2018 Jan 8;44(1):56-72.e4. doi: 10.1016/j.devcel.2017.12.014. Epub 2018 Jan 8.

Backbone

• Vector backbone: YIplac211
• Backbone size w/o insert (bp): 3797
• Total vector size (bp): 9165
• Vector type: Yeast Expression
• Selectable markers: URA3

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: Vps8 portion
• Species: S. cerevisiae (budding yeast)
• Entrez Gene: VPS8 (a.k.a. YAL002W, FUN15, VPL8, VPT8)
• Tag / Fusion Protein:
  • mCherry2Bx6 (C terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: SphI (not destroyed)
• 3′ cloning site: SacI (not destroyed)
• 5′ sequencing primer: M13 REVERSE
• 3′ sequencing primer: M13 FORWARD

Resource Information

• Addgene Notes:
  • Addgene Diagnostic Digest
• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Takara Bio Limited Use Label License (formerly Clontech)
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Please note that the Addgene verified sequence contains an additional transposase sequence compared the the depositor's reference map. This difference is not known to affect the plasmid's function.