YIplac211-msGFP-VPS21
(Plasmid #105265)

• Purpose: Plasmid for pop-in/pop-out replacement of Vps21 with msGFP-Vps21.
• Depositing Lab: Benjamin Glick
• Publication: Day et al Dev Cell. 2018 Jan 8;44(1):56-72.e4. doi: 10.1016/j.devcel.2017.12.014. Epub 2018 Jan 8.

Backbone

• Vector backbone: YIplac211
• Backbone size w/o insert (bp): 3797
• Total vector size (bp): 5997
• Vector type: Yeast Expression
• Selectable markers: URA3

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: Vps21
• Species: S. cerevisiae (budding yeast)
• Entrez Gene: VPS21 (a.k.a. YOR089C, VPS12, VPT12, YPT21, YPT51)
• Tag / Fusion Protein:
  • msGFP (N terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: SphI (not destroyed)
• 3′ cloning site: KpnI (not destroyed)
• 5′ sequencing primer: M13 REVERSE
• 3′ sequencing primer: M13 FORWARD

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Addgene's quality control identified R262G in URA3. The depositing lab states that this mutation should not affect selection.