Skip to main content
Addgene

sgPYM1
(Plasmid #105249)

Print

Full plasmid sequence is not available for this item.

Loading...

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 105249 Standard format: Plasmid sent in bacteria as agar stab 1 $85
Add to Cart

Backbone

  • Vector backbone
    pX458
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    sgRNA for PYM1
  • gRNA/shRNA sequence
    gcgtcaacacagcgacctga
  • Species
    H. sapiens (human)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    sgPYM1 was a gift from Geraldine Seydoux (Addgene plasmid # 105249 ; http://n2t.net/addgene:105249 ; RRID:Addgene_105249)

  • For your References section:

    Precision genome editing using synthesis-dependent repair of Cas9-induced DNA breaks. Paix A, Folkmann A, Goldman DH, Kulaga H, Grzelak MJ, Rasoloson D, Paidemarry S, Green R, Reed RR, Seydoux G. Proc Natl Acad Sci U S A. 2017 Nov 28. pii: 1711979114. doi: 10.1073/pnas.1711979114. 10.1073/pnas.1711979114 PubMed 29183983
Related items: