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pAP1892-1
(Plasmid #105247)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 105247 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pUC19
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Insertion of GFP11 at cut tagRFP 33bp downstream in Lamin A/C
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    800
  • Entrez Gene
    LMNA (a.k.a. CDCD1, CDDC, CMD1A, CMT2B1, EMD2, FPL, FPLD, FPLD2, HGPS, IDC, LDP1, LFP, LGMD1B, LMN1, LMNC, LMNL1, MADA, PRO1)

Cloning Information

  • Cloning method Ligation Independent Cloning
  • 5′ sequencing primer M13pUC-Fwd
  • 3′ sequencing primer M13pUC-Rev
    • (Common Sequencing Primers)

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pAP1892-1 was a gift from Geraldine Seydoux (Addgene plasmid # 105247 ; http://n2t.net/addgene:105247 ; RRID:Addgene_105247)

  • For your References section:

    Precision genome editing using synthesis-dependent repair of Cas9-induced DNA breaks. Paix A, Folkmann A, Goldman DH, Kulaga H, Grzelak MJ, Rasoloson D, Paidemarry S, Green R, Reed RR, Seydoux G. Proc Natl Acad Sci U S A. 2017 Nov 28. pii: 1711979114. doi: 10.1073/pnas.1711979114. 10.1073/pnas.1711979114 PubMed 29183983
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