p3xFlag-Alkbh4
(Plasmid #105240)

• Purpose: Expresses murine Alkbh4 containing an N-ternimal 3xFlag tag
• Depositing Lab: Douglas Feldman
• Publication: Kweon et al Mol Cell. 2019 Apr 5. pii: S1097-2765(19)30222-9. doi: 10.1016/j.molcel.2019.03.018.

Backbone

• Vector backbone: p3XFlag-7.1
• Backbone manufacturer: Sigma
• Backbone size w/o insert (bp): 4800
• Total vector size (bp): 5700
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Alkbh4
• Species: M. musculus (mouse)
• Insert Size (bp): 900
• Entrez Gene: Alkbh4 (a.k.a. 2010004B12Rik, Abj4)
• Promoter: CMV
• Tags / Fusion Proteins:
  • Flag (N terminal on backbone)
  • HA (C terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: EcoRI (not destroyed)
• 3′ cloning site: BamHI (not destroyed)
• 5′ sequencing primer: CMV-F

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Please note the F236L substitution in Alkbh4 has no measurable effect on catalytic activity and may be a mouse strain polymorphism.