PAX6 donor plasmid
(Plasmid #105239)

• Purpose: Knock in H2B-GFP into the human PAX6 locus
• Depositing Lab: Lorenz Studer
• Publication: Tchieu et al Cell Stem Cell. 2017 Sep 7;21(3):399-410.e7. doi: 10.1016/j.stem.2017.08.015.

Backbone

• Vector backbone: PUC19
• Backbone size w/o insert (bp): 2686
• Total vector size (bp): 7158
• Vector type: TALEN
• Selectable markers: Puromycin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): NEB Stable
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: PAX6 Homologous recombination ARMS with a P2A-H2B-GFP and Puromycin selection cassette
• Species: H. sapiens (human)
• Insert Size (bp): 4463

Cloning Information

• Cloning method: Gibson Cloning
• 5′ sequencing primer: N/A

Resource Information

• A portion of this plasmid was derived from a plasmid made by: H2B-GFP was a gift from Geoff Wahl (Addgene plasmid # 11680)
• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry