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Addgene

pBx-Spas-sgRNA-Kan
(Plasmid #105233)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 105233 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pBBR1
  • Backbone size w/o insert (bp) 5766
  • Total vector size (bp) 5847
  • Vector type
    Bacterial Expression, CRISPR, Synthetic Biology

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    S. pasteurianus sgRNA
  • Species
    S. pasteurianus
  • Insert Size (bp)
    81

Resource Information

  • Supplemental Documents
  • A portion of this plasmid was derived from a plasmid made by
    S. pasteurianus sgRNA was kindly provided by Rock et al. 2017. Programmable transcriptional repression in mycobacteria using an orthogonal CRISPR interference platform. Nat Microbiol 2:16274.

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pBx-Spas-sgRNA-Kan was a gift from Kristala Prather (Addgene plasmid # 105233 ; http://n2t.net/addgene:105233 ; RRID:Addgene_105233)

  • For your References section:

    A Robust CRISPR Interference Gene Repression System in Pseudomonas. Tan SZ, Reisch CR, Prather KLJ. J Bacteriol. 2018 Mar 12;200(7). pii: JB.00575-17. doi: 10.1128/JB.00575-17. Print 2018 Apr 1. 10.1128/JB.00575-17 PubMed 29311279
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