Ptrc::RpaA D53A
(Plasmid
#105229)
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Purposeinducible expression of unphosphorylatable variant of RpaA (D53A)
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 105229 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backboneNS2.2-targetting vector (into neutral site NS2.2)
- Backbone size w/o insert (bp) 6000
- Total vector size (bp) 10000
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Vector typeCyanobacteria cloning vector
Growth in Bacteria
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Bacterial Resistance(s)Gentamicin, 10 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert namerpaA driven by Ptrc promoter
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SpeciesNA
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Insert Size (bp)4000
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MutationD53 mutated to A
- Promoter ptrc
Cloning Information
- Cloning method Gibson Cloning
- 5′ sequencing primer -
- 3′ sequencing primer - (Common Sequencing Primers)
Resource Information
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A portion of this plasmid was derived from a plasmid made bygift from a lab member Joseph Markson
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
Ptrc::RpaA D53A was a gift from Erin O'Shea (Addgene plasmid # 105229 ; http://n2t.net/addgene:105229 ; RRID:Addgene_105229) -
For your References section:
Switching of metabolic programs in response to light availability is an essential function of the cyanobacterial circadian output pathway. Puszynska AM, O'Shea EK. Elife. 2017 Apr 21;6. pii: e23210. doi: 10.7554/eLife.23210. 10.7554/eLife.23210 PubMed 28430105
