pISH-Sncg
(Plasmid
#105217)
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Purposeto generate a riboprobe for in situ hybridazation
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 105217 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepGEM-T
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Backbone manufacturerPromega
- Backbone size w/o insert (bp) 3000
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Vector typeto ganerate riboprobe
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameSncg
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SpeciesM. musculus (mouse)
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Insert Size (bp)685
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Entrez GeneSncg (a.k.a. C79089, persyn)
Cloning Information
- Cloning method Unknown
- 5′ sequencing primer M13-rev
- 3′ sequencing primer M13-for (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Linearize with SpeI, use T7 RNA polymerase for IVT.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pISH-Sncg was a gift from Peter Mombaerts (Addgene plasmid # 105217 ; http://n2t.net/addgene:105217 ; RRID:Addgene_105217) -
For your References section:
Hierarchical deconstruction of mouse olfactory sensory neurons: from whole mucosa to single-cell RNA-seq. Saraiva LR, Ibarra-Soria X, Khan M, Omura M, Scialdone A, Mombaerts P, Marioni JC, Logan DW. Sci Rep. 2015 Dec 16;5:18178. doi: 10.1038/srep18178. 10.1038/srep18178 PubMed 26670777
