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Addgene

Gucy1b2-D-IRES-tauGFP-pA-ACNF TV
(Plasmid #105208)

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Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 105208 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pBluescript-II-KS+
  • Backbone size w/o insert (bp) 3000
  • Modifications to backbone
    PmeI and SgrAI sites were inserted between SacI and SacII. SacI and SacII sites were killed.
  • Vector type
    Mouse Targeting
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Gucy1b2-D-IRES-tauGFP-pA-ACNF
  • Alt name
    Gucy1b2
  • Species
    M. musculus (mouse)
  • Insert Size (bp)
    18196
  • Entrez Gene
    Gucy1b2 (a.k.a. B930025B03, GC-SB2)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamHI (not destroyed)
  • 3′ cloning site XhoI (not destroyed)
  • 5′ sequencing primer M13-20
  • 3′ sequencing primer M13-Reverse
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Linearize the TV with PmeI for electroporation.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    Gucy1b2-D-IRES-tauGFP-pA-ACNF TV was a gift from Peter Mombaerts (Addgene plasmid # 105208 ; http://n2t.net/addgene:105208 ; RRID:Addgene_105208)

  • For your References section:

    A Sensor for Low Environmental Oxygen in the Mouse Main Olfactory Epithelium. Bleymehl K, Perez-Gomez A, Omura M, Moreno-Perez A, Macias D, Bai Z, Johnson RS, Leinders-Zufall T, Zufall F, Mombaerts P. Neuron. 2016 Dec 21;92(6):1196-1203. doi: 10.1016/j.neuron.2016.11.001. Epub 2016 Dec 1. 10.1016/j.neuron.2016.11.001 PubMed 27916458
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