pLN540 (FuGW-G8p-IL12-P2A-CCL21-1Pe)
(Plasmid
#105201)
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PurposeModule 3 - GAL4 synthetic promoter (with eight binding sites) drives the expression of IL12 and CCL21 transcripts containing miR1-Bs(Pe)
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 105201 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backboneFUGW
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Vector typeMammalian Expression, Lentiviral, Synthetic Biology
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)NEB Stable
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Copy numberUnknown
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pLN540 (FuGW-G8p-IL12-P2A-CCL21-1Pe) was a gift from Timothy Lu (Addgene plasmid # 105201 ; http://n2t.net/addgene:105201 ; RRID:Addgene_105201) -
For your References section:
Synthetic RNA-Based Immunomodulatory Gene Circuits for Cancer Immunotherapy. Nissim L, Wu MR, Pery E, Binder-Nissim A, Suzuki HI, Stupp D, Wehrspaun C, Tabach Y, Sharp PA, Lu TK. Cell. 2017 Nov 16;171(5):1138-1150.e15. doi: 10.1016/j.cell.2017.09.049. Epub 2017 Oct 19. 10.1016/j.cell.2017.09.049 PubMed 29056342
