b2AR->M71-IRES-tauGFP-ACNF TV
(Plasmid
#105198)
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Purposetargeting vector to generate a replacement of CDS of M71 with CDS of mouse beta2-adrenergic receptor with expression of tauGFP.
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 105198 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepBluescript-II-SK
- Backbone size w/o insert (bp) 3000
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Vector typeMouse Targeting
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Selectable markersNeomycin (select with G418)
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)NEB Stable
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Copy numberHigh Copy
Gene/Insert
Cloning Information
- Cloning method Unknown
- 5′ sequencing primer T3
- 3′ sequencing primer T7 (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Linearize the TV with PmeI for electroporation.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
b2AR->M71-IRES-tauGFP-ACNF TV was a gift from Peter Mombaerts (Addgene plasmid # 105198 ; http://n2t.net/addgene:105198 ; RRID:Addgene_105198) -
For your References section:
The beta2-adrenergic receptor as a surrogate odorant receptor in mouse olfactory sensory neurons. Omura M, Grosmaitre X, Ma M, Mombaerts P. Mol Cell Neurosci. 2014 Jan;58:1-10. doi: 10.1016/j.mcn.2013.10.010. Epub 2013 Nov 6. 10.1016/j.mcn.2013.10.010 PubMed 24211702