pFA6a-kanMX6-3nmt1-mMaple3
(Plasmid #105167)

• Purpose: Yeast gene targeting
• Depositing Lab: Jian-Qiu Wu
• Publication: Liu et al Mol Biol Cell. 2016 Aug 15;27(16):2528-41. doi: 10.1091/mbc.E16-03-0156. Epub 2016 Jul 6.

Backbone

• Vector backbone: pFA6a
• Backbone size w/o insert (bp): 4170
• Vector type: Bacterial Expression, Yeast Expression
• Selectable markers: KanMX6 (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: kanMX6-3nmt1-mMaple3
• Alt name: mMaple3
• Alt name: 3nmt1
• Species: S. pombe (fission yeast)
• Promoter: 3nmt1
• Tag / Fusion Protein:
  • 3nmt1-mMaple3 (N terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: PacI (not destroyed)
• 3′ cloning site: AscI (not destroyed)

Resource Information

• Supplemental Documents:
  • 105167

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

GlySerGly are inserted before mMaple3 The sizes of backbone and inserts are approximate, and do not include the selectable marker.