pFA6a-mMaple3-kanMX6
(Plasmid
#105165)
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PurposeYeast gene targeting
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 105165 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepFA6a
- Backbone size w/o insert (bp) 4170
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Vector typeBacterial Expression, Yeast Expression
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Selectable markersKanMX6 (select with G418)
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namemMaple3-kanMX6
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Alt namemMaple3
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Tag
/ Fusion Protein
- GlySerGly-mMaple3 (C terminal on insert)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site PacI (not destroyed)
- 3′ cloning site AscI (not destroyed)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
The sizes of backbone and inserts are approximate, and do not include the selectable marker.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pFA6a-mMaple3-kanMX6 was a gift from Jian-Qiu Wu (Addgene plasmid # 105165 ; http://n2t.net/addgene:105165 ; RRID:Addgene_105165) -
For your References section:
Roles of the novel coiled-coil protein Rng10 in septum formation during fission yeast cytokinesis. Liu Y, Lee IJ, Sun M, Lower CA, Runge KW, Ma J, Wu JQ. Mol Biol Cell. 2016 Aug 15;27(16):2528-41. doi: 10.1091/mbc.E16-03-0156. Epub 2016 Jul 6. 10.1091/mbc.E16-03-0156 PubMed 27385337
Map uploaded by Addgene staff.
