pFA6a-tdTomato-hphMX6
(Plasmid
#105157)
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PurposeYeast gene targeting
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 105157 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepFA6a
- Backbone size w/o insert (bp) 4170
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Vector typeBacterial Expression, Yeast Expression
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Selectable markersHygromycin
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nametdTomato-hphMX6
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Alt nametandem dimer Tomato
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Insert Size (bp)1400
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Tag
/ Fusion Protein
- tdTomato (C terminal on insert)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site BglII (not destroyed)
- 3′ cloning site PmeI (not destroyed)
Resource Information
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Addgene Notes
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
The sizes of backbone and inserts are approximate, and do not include the selectable marker.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pFA6a-tdTomato-hphMX6 was a gift from Jian-Qiu Wu (Addgene plasmid # 105157 ; http://n2t.net/addgene:105157 ; RRID:Addgene_105157) -
For your References section:
Cooperation between Rho-GEF Gef2 and its binding partner Nod1 in the regulation of fission yeast cytokinesis. Zhu YH, Ye Y, Wu Z, Wu JQ. Mol Biol Cell. 2013 Oct;24(20):3187-204. doi: 10.1091/mbc.E13-06-0301. Epub 2013 Aug 21. 10.1091/mbc.E13-06-0301 PubMed 23966468