pFA6a-tdTomato-hphMX6
(Plasmid #105157)

• Purpose: Yeast gene targeting
• Depositing Lab: Jian-Qiu Wu
• Publication: Zhu et al Mol Biol Cell. 2013 Oct;24(20):3187-204. doi: 10.1091/mbc.E13-06-0301. Epub 2013 Aug 21.

Backbone

• Vector backbone: pFA6a
• Backbone size w/o insert (bp): 4170
• Vector type: Bacterial Expression, Yeast Expression
• Selectable markers: Hygromycin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: tdTomato-hphMX6
• Alt name: tandem dimer Tomato
• Insert Size (bp): 1400
• Tag / Fusion Protein:
  • tdTomato (C terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: BglII (not destroyed)
• 3′ cloning site: PmeI (not destroyed)

Resource Information

• Addgene Notes:
  • Addgene Diagnostic Digest

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Takara Bio Limited Use Label License (formerly Clontech)
• Industry Terms:
  • Not Available to Industry

Depositor Comments

The sizes of backbone and inserts are approximate, and do not include the selectable marker.