pFA6a-kanMX6-P41nmt1-mECitrine
(Plasmid
#105152)
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PurposeYeast gene targeting
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 105152 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepFA6a
- Backbone size w/o insert (bp) 4170
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Vector typeBacterial Expression, Yeast Expression
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Selectable markersKanMX6 (select with G418)
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namekanMX6-P41nmt1-mECitrine
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Alt namemonomeric enhanced Citrine
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Alt name41nmt1
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SpeciesS. pombe (fission yeast)
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Insert Size (bp)1900
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MutationF64L, Q69M, A206K
- Promoter 41nmt1
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Tag
/ Fusion Protein
- 41nmt1-mECitrine (N terminal on insert)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site PacI (not destroyed)
- 3′ cloning site AscI (not destroyed)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
The sizes of backbone and inserts are approximate, and do not include the selectable marker.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pFA6a-kanMX6-P41nmt1-mECitrine was a gift from Jian-Qiu Wu (Addgene plasmid # 105152 ; http://n2t.net/addgene:105152 ; RRID:Addgene_105152) -
For your References section:
The novel proteins Rng8 and Rng9 regulate the myosin-V Myo51 during fission yeast cytokinesis. Wang N, Lo Presti L, Zhu YH, Kang M, Wu Z, Martin SG, Wu JQ. J Cell Biol. 2014 May 12;205(3):357-75. doi: 10.1083/jcb.201308146. Epub 2014 May 5. 10.1083/jcb.201308146 PubMed 24798735