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pFA6a- mECitrine-kanMX6
(Plasmid #105150)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 105150 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pFA6a
  • Backbone size w/o insert (bp) 4170
  • Vector type
    Bacterial Expression, Yeast Expression
  • Selectable markers
    KanMX6 (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    mECitrine-kanMX6
  • Alt name
    monomeric enhanced Citrine
  • Insert Size (bp)
    720
  • Mutation
    F64L, Q69M, A206K
  • Tag / Fusion Protein
    • mECitrine (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site PacI (not destroyed)
  • 3′ cloning site AscI (not destroyed)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

mECitrine is brighter than mYFP at least for some proteins. mECitrine was constructed from mYFP (S65G, V68L, Q69K, S72A, T203Y, and A206K) by introducing the F64L and Q69M mutations. Also used in PMID: 22427686. The sizes of backbone and inserts are approximate, and do not include the selectable marker.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pFA6a- mECitrine-kanMX6 was a gift from Jian-Qiu Wu (Addgene plasmid # 105150 ; http://n2t.net/addgene:105150 ; RRID:Addgene_105150)

  • For your References section:

    Roles of putative Rho-GEF Gef2 in division-site positioning and contractile-ring function in fission yeast cytokinesis. Ye Y, Lee IJ, Runge KW, Wu JQ. Mol Biol Cell. 2012 Apr;23(7):1181-95. doi: 10.1091/mbc.E11-09-0800. Epub 2012 Feb 1. 10.1091/mbc.E11-09-0800 PubMed 22298427
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