pFA6a-3GFP-kanMX6
(Plasmid #105148)

• Purpose: Yeast gene targeting
• Depositing Labs: David Kovar, Thomas Pollard, Jian-Qiu Wu
• Publication: Wu et al J Cell Biol. 2006 Jul 31;174(3):391-402. doi: 10.1083/jcb.200602032. Epub 2006 Jul 24.

Backbone

• Vector backbone: pFA6a
• Backbone size w/o insert (bp): 4170
• Vector type: Bacterial Expression, Yeast Expression
• Selectable markers: KanMX6 (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: 3GFP-kanMX6
• Alt name: triple GFP
• Insert Size (bp): 2160
• Tag / Fusion Protein:
  • 3GFP (C terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: PacI (not destroyed)
• 3′ cloning site: AscI (not destroyed)

Resource Information

• Addgene Notes:
  • Addgene Diagnostic Digest

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

The sizes of backbone and inserts are approximate, and do not include the selectable marker.