pFA6a-kanMX6-P81nmt1-mEGFP
(Plasmid
#105143)
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PurposeYeast gene targeting
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 105143 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepFA6a
- Backbone size w/o insert (bp) 4170
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Vector typeBacterial Expression, Yeast Expression
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Selectable markersKanMX6 (select with G418)
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namekanMX6-P81nmt1-mEGFP
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Alt namemonomeric enhanced GFP
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Alt name81nmt1
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SpeciesS. pombe (fission yeast)
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Insert Size (bp)1900
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MutationA206K
- Promoter 81nmt1
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Tag
/ Fusion Protein
- 81nmt1-mEGFP (N terminal on insert)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site PacI (not destroyed)
- 3′ cloning site AscI (not destroyed)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
it is more thermal stable than GFP(S65T) The sizes of backbone and inserts are approximate, and do not include the selectable marker.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pFA6a-kanMX6-P81nmt1-mEGFP was a gift from Jian-Qiu Wu (Addgene plasmid # 105143 ; http://n2t.net/addgene:105143 ; RRID:Addgene_105143) -
For your References section:
alpha-Actinin and fimbrin cooperate with myosin II to organize actomyosin bundles during contractile-ring assembly. Laporte D, Ojkic N, Vavylonis D, Wu JQ. Mol Biol Cell. 2012 Aug;23(16):3094-110. doi: 10.1091/mbc.E12-02-0123. Epub 2012 Jun 27. 10.1091/mbc.E12-02-0123 PubMed 22740629