pFA6a-kanMX6-P81nmt1-mCFP
(Plasmid #105142)

• Purpose: Yeast gene targeting
• Depositing Labs: Thomas Pollard, Jian-Qiu Wu
• Publication: Wu et al Dev Cell. 2003 Nov;5(5):723-34.

Backbone

• Vector backbone: pFA6a
• Backbone size w/o insert (bp): 4170
• Vector type: Bacterial Expression, Yeast Expression
• Selectable markers: KanMX6 (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: kanMX6-P81nmt1-mCFP
• Alt name: monomeric CFP
• Alt name: 81nmt1
• Species: S. pombe (fission yeast)
• Insert Size (bp): 1900
• Mutation: A206K
• Promoter: 81nmt1
• Tag / Fusion Protein:
  • 81nmt1-mCFP (N terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: PacI (not destroyed)
• 3′ cloning site: AscI (not destroyed)

Resource Information

• Supplemental Documents:
  • 105142

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

The sizes of backbone and inserts are approximate, and do not include the selectable marker.