pFA6a-kanMX6-P3nmt1-mEGFP
(Plasmid #105137)

• Purpose: Yeast gene targeting
• Depositing Lab: Jian-Qiu Wu
• Publication: Laporte et al Mol Biol Cell. 2012 Aug;23(16):3094-110. doi: 10.1091/mbc.E12-02-0123. Epub 2012 Jun 27.

Backbone

• Vector backbone: pFA6a
• Backbone size w/o insert (bp): 4170
• Vector type: Bacterial Expression, Yeast Expression
• Selectable markers: KanMX6 (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: kanMX6-P3nmt1-mEGFP
• Alt name: monomeric enhanced GFP
• Alt name: 3nmt1
• Species: S. pombe (fission yeast)
• Insert Size (bp): 1900
• Mutation: A206K
• Promoter: 3nmt1
• Tag / Fusion Protein:
  • 3nmt1-mEGFP (N terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: PacI (not destroyed)
• 3′ cloning site: AscI (not destroyed)

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

it is more thermal stable than GFP(S65T) The sizes of backbone and inserts are approximate, and do not include the selectable marker.