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pFA6a-kanMX6-P3nmt1-mCFP
(Plasmid #105136)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 105136 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pFA6a
  • Backbone size w/o insert (bp) 4170
  • Vector type
    Bacterial Expression, Yeast Expression
  • Selectable markers
    KanMX6 (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    kanMX6-P3nmt1-mCFP
  • Alt name
    monomeric CFP
  • Alt name
    3nmt1
  • Species
    S. pombe (fission yeast)
  • Insert Size (bp)
    1900
  • Mutation
    A206K
  • Promoter 3nmt1
  • Tag / Fusion Protein
    • 3nmt1-mCFP (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site PacI (not destroyed)
  • 3′ cloning site AscI (not destroyed)

Resource Information

  • Supplemental Documents

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

The sizes of backbone and inserts are approximate, and do not include the selectable marker.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pFA6a-kanMX6-P3nmt1-mCFP was a gift from Thomas Pollard & Jian-Qiu Wu (Addgene plasmid # 105136 ; http://n2t.net/addgene:105136 ; RRID:Addgene_105136)

  • For your References section:

    Spatial and temporal pathway for assembly and constriction of the contractile ring in fission yeast cytokinesis. Wu JQ, Kuhn JR, Kovar DR, Pollard TD. Dev Cell. 2003 Nov;5(5):723-34. S1534-5807(03)00324-1 [pii] PubMed 14602073
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