pKE4-I-SceI-fabp10a:Xpt-β-cat, cryaa:Venus
(Plasmid #105127)

• Purpose: Used along with I-SceI enzyme to generate transgenic zebrafish expressing hepatocyte-specific Xenopus activated beta-catenin and lens-specific Venus fluorescent protein
• Depositing Labs: Kimberley Evason, Didier Stainier
• Publication: Evason et al PLoS Genet. 2015 Jul 2;11(7):e1005305. doi: 10.1371/journal.pgen.1005305. eCollection 2015 Jul.

Backbone

• Vector backbone: Unknown
• Vector type: Zebrafish transgenesis

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert 1

• Gene/Insert name: Zebrafish fabp10a promoter:Xenopus pt-beta-catenin coding sequence, polyA
• Species: X. laevis (frog)
• Insert Size (bp): 5280
• Entrez Gene: fabp10a (a.k.a. L-FABP, Lb-FABP, Lfabp, Zf-FABP10, fabp10, z-L-BABP, zgc:103719, zgc:92741)
• Promoter: fabp10a

Cloning Information for Gene/Insert 1

• Cloning method: Unknown
• 5′ sequencing primer: T7
• 3′ sequencing primer: SV40pA-R

Gene/Insert 2

• Gene/Insert name: Zebrafish cryaa promoter:Venus fluorescent protein coding sequence
• Species: D. rerio (zebrafish)
• Insert Size (bp): 1516
• Entrez Gene: cryaa (a.k.a. wu:fc06h04, zgc:92036)
• Promoter: cryaa (crystallin)

Cloning Information for Gene/Insert 2

• Cloning method: Unknown
• 5′ sequencing primer: unknown
• 3′ sequencing primer: T3

Resource Information

• A portion of this plasmid was derived from a plasmid made by: The Xenopus pt-beta-catenin sequence is derived from pCS2-XE49 (Randall Moon lab) The remainder of the sequence is derived from the pHD157 plasmid (Didier Stainier and Daniel Hesselson).

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry