pZMU-Dest
(Plasmid
#105089)
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Purpose(Empty Backbone) Yeast expression vector for Gateway cloning and SUS bridge assays
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 105089 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepZMU-Dest
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Vector typeYeast Expression
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Selectable markersURA3
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Tag
/ Fusion Protein
- myc (C terminal on backbone)
Growth in Bacteria
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Bacterial Resistance(s)Chloramphenicol and Spectinomycin, 25 & 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)ccdB Survival
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameNone
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Tag
/ Fusion Protein
- myc (C terminal on backbone)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pZMU-Dest was a gift from Christopher Grefen (Addgene plasmid # 105089 ; http://n2t.net/addgene:105089 ; RRID:Addgene_105089) -
For your References section:
The split-ubiquitin system for the analysis of three-component interactions. Grefen C. Methods Mol Biol. 2014;1062:659-78. doi: 10.1007/978-1-62703-580-4_34. 10.1007/978-1-62703-580-4_34 PubMed 24057391
Map uploaded by the depositor.
