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Addgene

pXNubA22-Dest
(Plasmid #105087)

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 105087 Standard format: Plasmid sent in bacteria as agar stab 1 $85

Backbone

  • Vector backbone
    pXNubA22-Dest
  • Vector type
    Yeast Expression
  • Selectable markers
    TRP1
  • Tag / Fusion Protein
    • NubA (C terminal on backbone)

Growth in Bacteria

  • Bacterial Resistance(s)
    Chloramphenicol and Ampicillin, 25 & 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    ccdB Survival
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    None
  • Tag / Fusion Protein
    • NubA (C terminal on backbone)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pXNubA22-Dest was a gift from Christopher Grefen (Addgene plasmid # 105087 ; http://n2t.net/addgene:105087 ; RRID:Addgene_105087)
  • For your References section:

    The determination of protein-protein interactions by the mating-based split-ubiquitin system (mbSUS). Grefen C, Obrdlik P, Harter K. Methods Mol Biol. 2009;479:217-33. doi: 10.1007/978-1-59745-289-2_14. 10.1007/978-1-59745-289-2_14 PubMed 19083185