pXN21-Dest
(Plasmid
#105086)
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Purpose(Empty Backbone) Yeast mating-based Split Ubiquitin Assay, prey vector for Gateway cloning
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 105086 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepXN21-Dest
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Vector typeYeast Expression
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Selectable markersTRP1
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Tag
/ Fusion Protein
- NubG (C terminal on backbone)
Growth in Bacteria
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Bacterial Resistance(s)Chloramphenicol and Ampicillin, 25 & 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)ccdB Survival
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameNone
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Tag
/ Fusion Protein
- NubG (C terminal on backbone)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pXN21-Dest was a gift from Christopher Grefen (Addgene plasmid # 105086 ; http://n2t.net/addgene:105086 ; RRID:Addgene_105086) -
For your References section:
Split-ubiquitin system for identifying protein-protein interactions in membrane and full-length proteins. Grefen C, Lalonde S, Obrdlik P. Curr Protoc Neurosci. 2007 Oct;Chapter 5:Unit 5.27. doi: 10.1002/0471142301.ns0527s41. 10.1002/0471142301.ns0527s41 PubMed 18428659
