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Addgene

pNX32-Dest
(Plasmid #105083)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 105083 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pNX32-Dest
  • Vector type
    Yeast Expression
  • Selectable markers
    TRP1
  • Tag / Fusion Protein
    • NubG (N terminal on backbone)

Growth in Bacteria

  • Bacterial Resistance(s)
    Chloramphenicol and Ampicillin, 25 & 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    ccdB Survival
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    None
  • Tag / Fusion Protein
    • NubG (N terminal on backbone)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pNX32-Dest was a gift from Christopher Grefen (Addgene plasmid # 105083 ; http://n2t.net/addgene:105083 ; RRID:Addgene_105083)

  • For your References section:

    Split-ubiquitin system for identifying protein-protein interactions in membrane and full-length proteins. Grefen C, Lalonde S, Obrdlik P. Curr Protoc Neurosci. 2007 Oct;Chapter 5:Unit 5.27. doi: 10.1002/0471142301.ns0527s41. 10.1002/0471142301.ns0527s41 PubMed 18428659
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