pMetOYC-Dest
(Plasmid
#105082)
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Purpose(Empty Backbone) Yeast mating-based Split Ubiquitin Assay, bait vector for Gateway cloning
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 105082 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepMetOYC-Dest
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Vector typeYeast Expression
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Selectable markersLEU2
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Tags
/ Fusion Proteins
- mOST4 (N terminal on backbone)
- Cub-PLV (C terminal on backbone)
Growth in Bacteria
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Bacterial Resistance(s)Chloramphenicol and Ampicillin, 25 & 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)ccdB Survival
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameNone
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Tags
/ Fusion Proteins
- mOST4 (N terminal on backbone)
- Cub-PLV (C terminal on backbone)
Resource Information
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Supplemental Documents
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Article Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pMetOYC-Dest was a gift from Christopher Grefen (Addgene plasmid # 105082 ; http://n2t.net/addgene:105082 ; RRID:Addgene_105082) -
For your References section:
Techniques for the Analysis of Protein-Protein Interactions in Vivo. Xing S, Wallmeroth N, Berendzen KW, Grefen C. Plant Physiol. 2016 Jun;171(2):727-58. doi: 10.1104/pp.16.00470. Epub 2016 Apr 25. 10.1104/pp.16.00470 PubMed 27208310
Map uploaded by the depositor.
