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Addgene

pNXgate32-3HA
(Plasmid #105076)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 105076 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pNXgate32-3HA
  • Vector type
    Yeast Expression
  • Selectable markers
    TRP1 ; G418
  • Tags / Fusion Proteins
    • NubG (N terminal on backbone)
    • 3xHA (C terminal on backbone)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    None
  • Tags / Fusion Proteins
    • NubG (N terminal on backbone)
    • 3xHA (C terminal on backbone)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pNXgate32-3HA was a gift from Wolf Frommer (Addgene plasmid # 105076 ; http://n2t.net/addgene:105076 ; RRID:Addgene_105076)

  • For your References section:

    K+ channel interactions detected by a genetic system optimized for systematic studies of membrane protein interactions. Obrdlik P, El-Bakkoury M, Hamacher T, Cappellaro C, Vilarino C, Fleischer C, Ellerbrok H, Kamuzinzi R, Ledent V, Blaudez D, Sanders D, Revuelta JL, Boles E, Andre B, Frommer WB. Proc Natl Acad Sci U S A. 2004 Aug 17;101(33):12242-7. Epub 2004 Aug 6. 10.1073/pnas.0404467101 PubMed 15299147
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