pMetYCgate
(Plasmid
#105074)
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Purpose(Empty Backbone) Yeast mating-based Split Ubiquitin Assay, bait vector for in vivo cloning
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 105074 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepMetYCgate
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Vector typeYeast Expression
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Selectable markersLEU2 ; G418
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Tag
/ Fusion Protein
- Cub-PLV (C terminal on backbone)
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameNone
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Tag
/ Fusion Protein
- Cub-PLV (C terminal on backbone)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pMetYCgate was a gift from Wolf Frommer (Addgene plasmid # 105074 ; http://n2t.net/addgene:105074 ; RRID:Addgene_105074) -
For your References section:
K+ channel interactions detected by a genetic system optimized for systematic studies of membrane protein interactions. Obrdlik P, El-Bakkoury M, Hamacher T, Cappellaro C, Vilarino C, Fleischer C, Ellerbrok H, Kamuzinzi R, Ledent V, Blaudez D, Sanders D, Revuelta JL, Boles E, Andre B, Frommer WB. Proc Natl Acad Sci U S A. 2004 Aug 17;101(33):12242-7. Epub 2004 Aug 6. 10.1073/pnas.0404467101 PubMed 15299147
