Drd1a->M71-IRES-tauGFP ACNF TV
(Plasmid
#105073)
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PurposeTargeting vector: the coding sequence of M71 is replaced by the sequence encoding amino acids 1-447 of Drd1a and an IRES-tauGFP followed by ACNF cassette
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 105073 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepBlueScript II SK
- Backbone size w/o insert (bp) 3000
- Total vector size (bp) 19000
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Vector typeMouse Targeting
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Selectable markersNeomycin (select with G418)
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)NEB Stable
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameDrd1a->M71-IRES-tauGFP-ACNF
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Alt nameDrd1a
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Alt nameM71
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Alt nameolfr151
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SpeciesM. musculus (mouse)
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Insert Size (bp)16000
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MutationDrd1a coding sequence followed by IRES-tauGFP ACNF
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GenBank IDNM_010073 NM_207664
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Entrez GeneDrd1 (a.k.a. C030036C15Rik, Drd-1, Drd1a, Gpcr15)
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Entrez GeneOlfr151 (a.k.a. M71, MOR171-2)
Cloning Information
- Cloning method Unknown
- 5′ sequencing primer M13
- 3′ sequencing primer M13r (Common Sequencing Primers)
Resource Information
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A portion of this plasmid was derived from a plasmid made byA generic M71 targeting vector, which is described previously, was used
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
References:
-Feinstein, P., Bozza, T., Rodriguez, I., Vassalli, A., Mombaerts, P., 2004. Axon guidance of mouse olfactory sensory neurons by odorant receptors and the β2 adrenergic receptor. Cell 117, 833–846.
-Omura, M., Grosmaitre, X., Ma, M., Mombaerts, P., 2014. The β2-adrenergic receptor as a surrogate odorant receptor in mouse olfactory sensory neurons. Mol. Cell. Neurosci. 58, 1-10.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
Drd1a->M71-IRES-tauGFP ACNF TV was a gift from Peter Mombaerts (Addgene plasmid # 105073 ; http://n2t.net/addgene:105073 ; RRID:Addgene_105073) -
For your References section:
G-protein coupled receptors Mc4r and Drd1a can serve as surrogate odorant receptors in mouse olfactory sensory neurons. Katidou M, Grosmaitre X, Lin J, Mombaerts P. Mol Cell Neurosci. 2018 Apr;88:138-147. doi: 10.1016/j.mcn.2018.01.010. Epub 2018 Jan 31. 10.1016/j.mcn.2018.01.010 PubMed 29407371