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Purposehost strain for phage assisted continuous evolution
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Depositing Lab
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Sequence Information
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Full plasmid sequence is not available for this item.
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Bacterial Strain | 105063 | Bacteria in agar stab | 1 | $85 |
Backbone
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Vector backbonenone
Growth in Bacteria
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Bacterial Resistance(s)Streptomycin and Tetracycline
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Growth Temperature37°C
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Growth Strain(s)S1030
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Copy numberLow Copy
Gene/Insert
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Gene/Insert namenone
Resource Information
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Supplemental Documents
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Articles Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
S1030 Genotype:
F’ proA+B+ Δ(lacIZY) zzf::Tn10 lacIQ1 PN25-tetR luxCDE / endA1 recA1 galE15 galK16 nupG rpsL ΔlacIZYA araD139 Δ(ara,leu)7697 mcrA Δ(mrr-hsdRMS-mcrBC) proBA::pir116 araE201 ΔrpoZ Δflu ΔcsgABCDEFG ΔpgaC λ–
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
S1030 was a gift from David Liu (Addgene plasmid # 105063) -
For your References section:
Negative selection and stringency modulation in phage-assisted continuous evolution. Carlson JC, Badran AH, Guggiana-Nilo DA, Liu DR. Nat Chem Biol. 2014 Mar;10(3):216-22. doi: 10.1038/nchembio.1453. Epub 2014 Feb 2. 10.1038/nchembio.1453 PubMed 24487694