pcDNA3.1 human A3F-CTD
(Plasmid #105052)

• Purpose: Overexpression of APOBEC3F C-terminal domain in mammalian cell lines
• Depositing Lab: Carsten Münk
• Publication: Jaguva Vasudevan et al J Mol Biol. 2017 Apr 21;429(8):1171-1191. doi: 10.1016/j.jmb.2017.03.015. Epub 2017 Mar 16.

Backbone

• Vector backbone: pcDNA3.1
• Backbone manufacturer: Invitrogen
• Backbone size w/o insert (bp): 5428
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: APOBEC3F (A3F) C-terminal domain
• Species: H. sapiens (human)
• Insert Size (bp): 603
• Mutation: it contains only C terminal domain of A3F
• Entrez Gene: APOBEC3F (a.k.a. A3F, ARP8, BK150C2.4.MRNA, KA6)
• Promoter: CMV
• Tag / Fusion Protein:
  • HA tag (C terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: HindIII (unknown if destroyed)
• 3′ cloning site: EcoRI (unknown if destroyed)
• 5′ sequencing primer: 5'-CGCAAATGGGCGGTAGGCGTG
• 3′ sequencing primer: 5'-TAGAAGGCACAGTCGAGG

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry