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pcDNA3.1 human A3C.D99E
(Plasmid #105049)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 105049 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pcDNA3.1
  • Backbone manufacturer
    Invitrogen
  • Backbone size w/o insert (bp) 5428
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    APOBEC3C (A3C)
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    600
  • Mutation
    Aspartate 99 to glutamate
  • Entrez Gene
    APOBEC3C (a.k.a. A3C, APOBEC1L, ARDC2, ARDC4, ARP5, PBI, bK150C2.3)
  • Promoter CMV
  • Tag / Fusion Protein
    • HA tag (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site unknown (unknown if destroyed)
  • 3′ cloning site unknown (unknown if destroyed)
  • 5′ sequencing primer 5'-CGCAAATGGGCGGTAGGCGTG
  • 3′ sequencing primer 5'-TAGAAGGCACAGTCGAGG
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pcDNA3.1 human A3C.D99E was a gift from Carsten Münk (Addgene plasmid # 105049 ; http://n2t.net/addgene:105049 ; RRID:Addgene_105049)

  • For your References section:

    Enhancing the Catalytic Deamination Activity of APOBEC3C Is Insufficient to Inhibit Vif-Deficient HIV-1. Jaguva Vasudevan AA, Hofmann H, Willbold D, Haussinger D, Koenig BW, Munk C. J Mol Biol. 2017 Apr 21;429(8):1171-1191. doi: 10.1016/j.jmb.2017.03.015. Epub 2017 Mar 16. 10.1016/j.jmb.2017.03.015 PubMed 28315663
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