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pKLV2-U6gRNA5(Tsc2-g1)-PGKpuroBFP-W
(Plasmid #105039)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 105039 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pKLV2-U6gRNA5(BbsI)-PGKpuroBFP-W
  • Backbone manufacturer
    Yusa Lab
  • Vector type
    Mammalian Expression, Lentiviral, CRISPR
  • Selectable markers
    Puromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Tsc2
  • gRNA/shRNA sequence
    CTCATACACTCGAGTGGCG
  • Species
    M. musculus (mouse)
  • Entrez Gene
    Tsc2 (a.k.a. Nafld, Tcs2)
  • Promoter U6

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BbsI (destroyed during cloning)
  • 3′ cloning site BbsI (destroyed during cloning)
  • 5′ sequencing primer LKO.1 5'
    • (Common Sequencing Primers)

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pKLV2-U6gRNA5(Tsc2-g1)-PGKpuroBFP-W was a gift from Kosuke Yusa (Addgene plasmid # 105039 ; http://n2t.net/addgene:105039 ; RRID:Addgene_105039)

  • For your References section:

    Genome-wide CRISPR-KO Screen Uncovers mTORC1-Mediated Gsk3 Regulation in Naive Pluripotency Maintenance and Dissolution. Li M, Yu JSL, Tilgner K, Ong SH, Koike-Yusa H, Yusa K. Cell Rep. 2018 Jul 10;24(2):489-502. doi: 10.1016/j.celrep.2018.06.027. 10.1016/j.celrep.2018.06.027 PubMed 29996108
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