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pSF4 TetCMV intron 20xGCN4 Renilla Rh1 STOP 24xMS2v5 SV40 CTE polyA
(Plasmid #105000)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 105000 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pSF4
  • Vector type
    Mammalian Expression, Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Renilla luciferase
  • Species
    Renilla reniformis
  • Insert Size (bp)
    930
  • Promoter TetCMV
  • Tags / Fusion Proteins
    • 24xGCN4 repeats (SunTag) (N terminal on insert)
    • PreScission protease site-Rh1-RILPL2 (C terminal on insert)
    • 24xPP7v3 stem loops in 3'UTR
    • xrRNA1-xrRNA2 in 3'UTR
    • 24xMS2v5 stem loops in 3'UTR

Cloning Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Note plasmid contains 24xGCN4 repeats and not 20xGCN4 repeats as indicated in the name.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pSF4 TetCMV intron 20xGCN4 Renilla Rh1 STOP 24xMS2v5 SV40 CTE polyA was a gift from Jeffrey Chao (Addgene plasmid # 105000 ; http://n2t.net/addgene:105000 ; RRID:Addgene_105000)

  • For your References section:

    Single-Molecule Quantification of Translation-Dependent Association of mRNAs with the Endoplasmic Reticulum. Voigt F, Zhang H, Cui XA, Triebold D, Liu AX, Eglinger J, Lee ES, Chao JA, Palazzo AF. Cell Rep. 2017 Dec 26;21(13):3740-3753. doi: 10.1016/j.celrep.2017.12.008. 10.1016/j.celrep.2017.12.008 PubMed 29281824
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