phage UbiC scAB-GFP
(Plasmid
#104998)
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PurposeLentiviral vector expressing sequence-optimized single chain Antibody-GFP fusion protein for SunTag labeling
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Depositing Lab
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Sequence Information
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Sequences (1) — Accept Affinity Reagent Sequence Policy
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Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 104998 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepHAGE-UbiC
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Vector typeMammalian Expression, Bacterial Expression, Lentiviral
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)NEB Stable
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namesingle chain Antibody against GCN4
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SpeciesSynthetic
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Insert Size (bp)741
- Promoter UbiC
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Tags
/ Fusion Proteins
- HA tag (C terminal on insert)
- sfGFP (C terminal on insert)
- GB1 (C terminal on insert)
Cloning Information
- Cloning method Unknown
- 5′ sequencing primer GTGTGGCACAGCTAGTTCC (Common Sequencing Primers)
Resource Information
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Articles Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
phage UbiC scAB-GFP was a gift from Jeffrey Chao (Addgene plasmid # 104998 ; http://n2t.net/addgene:104998 ; RRID:Addgene_104998) -
For your References section:
Single-Molecule Quantification of Translation-Dependent Association of mRNAs with the Endoplasmic Reticulum. Voigt F, Zhang H, Cui XA, Triebold D, Liu AX, Eglinger J, Lee ES, Chao JA, Palazzo AF. Cell Rep. 2017 Dec 26;21(13):3740-3753. doi: 10.1016/j.celrep.2017.12.008. 10.1016/j.celrep.2017.12.008 PubMed 29281824