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Addgene

pACYCduet-mxLOX2-EH
(Plasmid #104979)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 104979 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pACYCDuet
  • Backbone manufacturer
    Novagen
  • Backbone size w/o insert (bp) 4008
  • Total vector size (bp) 6914
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Chloramphenicol, 25 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    Unknown

Gene/Insert 1

  • Gene/Insert name
    MXAN_1744
  • Alt name
    mxLOX2
  • Species
    Myxococcus xanthus
  • Insert Size (bp)
    2028
  • GenBank ID
    ABF 88826.1
  • Entrez Gene
    MXAN_1744 (a.k.a. MXAN_1744)
  • Promoter T7 promoter

Cloning Information for Gene/Insert 1

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRI (not destroyed)
  • 3′ cloning site HindIII (not destroyed)
  • 5′ sequencing primer GAATTCATGACTGTCGAGTACAAAC
  • 3′ sequencing primer TACAAGCTTTCAGACGGTGATGCCG
    • (Common Sequencing Primers)

Gene/Insert 2

  • Gene/Insert name
    MXAN_1644
  • Alt name
    mxEH
  • Species
    Myxococcus xanthus
  • Insert Size (bp)
    957
  • GenBank ID
    ABF 91519.1
  • Entrez Gene
    MXAN_1644 (a.k.a. MXAN_1644)
  • Promoter T7 promoter

Cloning Information for Gene/Insert 2

  • Cloning method Restriction Enzyme
  • 5′ cloning site NdeI (not destroyed)
  • 3′ cloning site XhoI (not destroyed)
  • 5′ sequencing primer GCGCATATGGCTGACATCACGCATCGAACCGT
  • 3′ sequencing primer GCGCTCGAGTCAGGCCGGCAGCTTCTTCA
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Addgene sequencing data found D273G in the MXAN_1744 translation, that does not affect protein function

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pACYCduet-mxLOX2-EH was a gift from Deokkun Oh (Addgene plasmid # 104979 ; http://n2t.net/addgene:104979 ; RRID:Addgene_104979)
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